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Fig. 7 | BMC Biochemistry

Fig. 7

From: Respiration and substrate transport rates as well as reactive oxygen species production distinguish mitochondria from brain and liver

Fig. 7

NADH-linked substrate transport is increased in brain compared with liver mitochondria. a The activity of the malate-aspartate shuttle was assessed by following the decrease in absorbance of NADH (0.14 mM) at 340 nM. After addition of mitochondria, baseline decrease in absorbance was recorded. Subsequently, further decrease of absorbance, indicative of malate-aspartate shuttle activity, was recorded after the addition of malate and glutamate. The data shown represent the average of three independent experiments performed in at least duplicate. b State 3 respiration supported by the complex I substrates glutamate and malate in the presence of ADP, with the stepwise (1 mM increments) addition of the malate aspartate shuttle inhibitor AOA. Traces were compiled from of three independent experiments performed in duplicate. c Brain mitochondrial state 3 respiration was determined in the presence of increasing concentrations of AOA in comparison with liver mitochondria in state 3 respiration without inhibition by AOA. Bars are representative of three independent experiments performed in duplicate. In 7b liver and brain mitochondria were compared for an individual dose of AOA. In 7c brain mitochondria at each AOA dose was compared to untreated liver mitochondria. Notations, ****-p < 0.0001, ***-p < 0.001, **-p < 0.01, N.S-Not significant

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