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Fig. 6 | BMC Biochemistry

Fig. 6

From: Respiration and substrate transport rates as well as reactive oxygen species production distinguish mitochondria from brain and liver

Fig. 6

Mitochondrial membrane potential is not different comparing brain and liver mitochondria and is equally affected by ETC. inhibitors in both mitochondrial populations. Mitochondrial membrane potential was qualitatively assessed for brain and liver mitochondria in respiratory state 2 utilizing glutamate and malate (a and b, respectively) or succinate (c and d, respectively). Mitochondria (150 μg) were added where indicated and membrane potential was recorded. At three minutes, 10 μM rotenone (black), 10 μM DPI (red), 10 μM CMB (green), 10 μM myxothiazol (orange), 10 μg/mL antimcyin A (blue), or 0.4 μM FCCP (gray) were added. Traces are representative of three replicates

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BMC Biochemistry

ISSN: 1471-2091

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