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Fig. 2 | BMC Biochemistry

Fig. 2

From: Respiration and substrate transport rates as well as reactive oxygen species production distinguish mitochondria from brain and liver

Fig. 2

Response of reactive oxygen species production to ETC inhibitors differs between brain and liver mitochondria. Brain (a) and liver (b) mitochondrial ROS production was assessed utilizing the complex I substrates glutamate and malate. Basal ROS production was recorded for three minutes. After this time, DPI (10 μM), CMB (10 μM), rotenone (10 μM), rotenone + DPI (10 μM each), rotenone + CMB (10 μM each), antimycin A (10 μM), or myxothiazol (10 μM) was added to the reaction and ROS production was recorded for an additional two minutes. Brain (c) and liver (d) mitochondrial ROS production was assessed utilizing the complex II substrate succinate. Basal respiration was recorded for three minutes. After this time, DPI (10 μM), CMB (10 μM), rotenone (10 μM), antimcyin A (10 μM), myxothiazol (10 μM), or rotenone + myxothiazol (10 μM each; liver only) was added to the reaction, and ROS production was recorded for an additional two minutes. 150 μg of isolated mitochondria was used for each experiment. For each comparison, * vs. § < 0.05; * vs. # < 0.001; * vs. † < 0.01; § vs. # < 0.01, § vs. † < 0.01; # vs. † < 0.05. For each condition, n ≥ 3

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