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Table 1 Purification of recombinant R. arrhizus cyt c

From: Purification and characterization of a cytochrome c with novel caspase-3 activation activity from the pathogenic fungus Rhizopus arrhizus

Purification step

Total Cyt c (mg)

Purity (OD410/280)

Recovery (%)

Fold purification

Lysate

43.9 ± 1.2

0.223 ± 0.005

100

1.00

Ammonium Sulphate (Precipitation)

30.8 ± 0.7

0.306 ± 0.001

70.2

1.37

Dialysis

20.7 ± 0.2

0.440 ± 0.001

47.2

1.97

Cation exchange

12.4 ± 0.3

3.91 ± 0.10

28.2

17.50

Gel filtration

9.12 ± 0.13

4.26 ± 0.03

19.7

19.10

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BMC Biochemistry

ISSN: 1471-2091

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