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Fig. 1 | BMC Biochemistry

Fig. 1

From: Purification and characterization of a cytochrome c with novel caspase-3 activation activity from the pathogenic fungus Rhizopus arrhizus

Fig. 1

a Elution profile of recombinant R. arrhizus cyt c on a Hi-Trap SP Sepharose column (5 ml). The bound protein was eluted with an increasing NaCl gradient over 7 column volumes. Peak fractions containing cyt c protein is indicated by *. b Elution profile of the pooled HiTrap SP Sepharose peak fractions loaded onto a Superdex 200 GL column. Cyt c elution was followed using 410 nm absorption. Cyt c protein elutes as two unresolved peaks (in inset peak 1 and 2). c Coomassie stained 15 % SDS-PAGE gel loaded (lane 1 and 2) with the peak 1 and 2 from the Superdex 200 GL column, lane 3 = marker proteins. Cyt c band moves close to the 14 kDa marker band

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