Identification of inhibitors that target dual-specificity phosphatase 5 provide new insights into the binding requirements for the two phosphate pockets

Table 2 DUSP5 PD(WT) pNPP enzymatic assay data for Z’ calculation (96 well plates)

a) End-point assay after 90 min
	Positive control	Negative control
Mean	0.006	0.269
Standard deviation	0.004	0.02
b) Kinetic (continuous) assay over 5 min
	Positive control	Negative control
Mean	−0.035	3.28
Standard deviation	0.091	0.193

ISSN: 1471-2091