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Fig. 5 | BMC Biochemistry

Fig. 5

From: Identification of inhibitors that target dual-specificity phosphatase 5 provide new insights into the binding requirements for the two phosphate pockets

Fig. 5

Effect of Detergent on Suramin Inhibition. a NTS IC50 measurement in the presence and absence of 0.5 % Triton, showing no detergent-effect on inhibition of DUSP5 PD(WT) pNPP phosphatase activity. b Suramin IC50 measurement in the presence and absence of 0.5 % Triton X-100 shows a loss of some inhibitory capability in the presence of detergent. c Effect of increasing detergent levels (Triton X-100) on rate of DUSP5 PD(WT) in the presence of a fixed concentrations of inhibitor and substrate (at 1 μM suramin and 5 mM pNPP). Detergent removes some, but not all, of suramin’s inhibitory effect, showing a plateau level 30 % inhibition

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