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Table 1 Experimental characterization of Symmetrin proteins. Each column displays the results of a single experiment performed on all Symmetrin designs. Each row displays the results of all experiments performed on a single Symmetrin design. The Symmetrin-1 and Symmetrin-3 designs displayed the most noteworthy characteristics. Cells left blank represent experiments not performed

From: Design of symmetric TIM barrel proteins from first principles

Protein

Expression

Solubility

CD spectrum

Tm

FPLC

1D NMR

Symmetrin-1

10 mg/l

soluble

alpha-beta

44 °C

monomeric

well resolved

Symmetrin-2

10 mg/l

soluble

alpha-beta

44 °C

monomeric

 

Symmetrin-3

10 mg/l

soluble

alpha-beta

63 °C

oligomeric

poorly-resolved

Symmetrin-4

3 mg/l

insoluble

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