Fig. 6
From: Design of symmetric TIM barrel proteins from first principles
Experimental characterization of Symmetrin-1 and Symmetrin-3. Figures (a,b,c) show experimental results for Symmetrin-1. a The far-ultraviolet circular dichroism (CD) spectrum is shown, displaying prominent alpha-beta characteristics. b Temperature denaturation recorded at 222 nm from 10-95 °C. The denaturation curve appears sigmoidal. c Gel filtration (FPLC) data, showing a prominent peak at 62.43 ml. Elution volumes for markers are shown in grey, while their corresponding molecular weights are shown in red. Figures (d,e,f) show experimental results for Symmetrin-3. d The far-ultraviolet CD spectrum is shown, displaying prominent alpha-beta characteristics. e Temperature denaturation recorded at 222 nm from 10-95 °C. The denaturation curve appears sigmoidal. Note that the protein appears more stable than Symmetrin-1. f Gel filtration (FPLC) data, showing a many peaks from 50.38 ml to 64.18 ml. Elution volumes for markers are shown in grey, while their corresponding molecular weights are shown in red