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Fig. 4 | BMC Biochemistry

Fig. 4

From: Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations

Fig. 4

Excitation and emission spectra of purified GPFUV variants. a Purified wtGFPUV and deletion mutants, resolved on 12 % SDS-PAGE. M, marker. Lane 1 to 6, wtGFPUV, C48Δ, P75Δ, P75/D76➔H, E172Δ and S175/V176➔F, respectively. b Normalized excitation spectra. c Normalized emission spectra. Note that the spectra of these variants are largely overlapped

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BMC Biochemistry

ISSN: 1471-2091

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