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Fig. 4 | BMC Biochemistry

Fig. 4

From: Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a

Fig. 4

Kv1.3-eGFP trafficking after siRNA mediated knockdown of Sec24. Kv1.3-eGFP trafficking was examined after the knockdown of Sec24 isoforms (as indicated) in the presences of the membranous ER resident protein Sec61β tagged with the mCherry fluorophore (Sec61β-mCherry). Cellular nuclei were stained with DAPI. The wild-type (wt) trafficking profile is similar to the trafficking profile of Sec24c and Sec24cd knockdown conditions. An altered trafficking profile is seen in Sec24a, Sec24b, Sec24ab, and Sec24abcd conditions. Interestingly, there is also an altered trafficking profile in the Sec24d condition, but the Kv1.3-eGFP signal does not overlap well with the Sec61β protein. Line scans of each fluorescent channel are shown. The white arrow in the merged image represents the placement and direction of the line scan. Scale bar = 5 μm

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