Fig. 5

Effects of altered POLD1 expression on DNA damage in HEK293 cells. a HEK293 cells were transfected with the indicated vectors and treated with 150 μM H₂O₂ for 5 min in the dark. Comet assay was then performed, and images were acquired using a fluorescence microscope to show DNA fragment migration patterns. b Quantification of the tail moments from the comet assay shown in (a). *P < 0.01, vs. the negative control (shControl or shControl + H₂O₂)