Fig. 5
From: Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides
2-5A nuclease assay using crude protein extracts. 10 μg of crude protein extracts of HeLa cells transfected with PDE12ΔmTP-His, red-fluorescent protein (DsRed), empty-vector pTriEx-3 Neo (EV) or mock-transfected (−) were incubated with (a) A(pA)3 tetramer core, (b) A(pA)4 pentamer core or (c) A(pA)5 hexamer core for one hour at 37 °C, and the products resolved by Mono Q chromatography. Note that each figure (a-c) includes four superimposed chromatograms: blue curves PDE12, yellow curves DsRed, purple curves EV and green curves -. The 2-5A core molecules are numbered according to length with 2 specifying the ApA dimer core, 3 the A(pA)2 trimer core and so forth. Brown curves: Experimental salt gradients. The chromatograms were obtained with 254 nm as the absorbance wavelength. mAU, milli-absorbance unit and mS/cm, milliSiemens/centimeter