Fig. 4
From: Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides
2-5A nuclease assay using a purified protein. 1.2 μg of purified human recombinant PDE12ΔmTP-His enzyme was incubated in reactions with (a) A(pA)3 tetramer core, (b) A(pA)4 pentamer core or (c) the A(pA)5 hexamer core for 1 h at 37 °C, and the products resolved by Mono Q chromatography. Each figure (a-c) includes two chromatograms that were superimposed. Blue curves: Profiles from reactions in which the enzyme was used, and red curves: Profiles from reactions where the enzyme was omitted. The 2-5A core molecules were numbered according to length with 2 specifying the ApA dimer core, 3 the A(pA)2 trimer core and so forth. Brown curves: Experimental salt gradients. Chromatograms were obtained with 254 nm as the absorbance wavelength. mAU, milli-absorbance unit and mS/cm, milliSiemens/centimeter. This figure has been reproduced from [19] with courtesy of Biochimie, Elsevier