Fig. 2
From: Enzyme assays for synthesis and degradation of 2-5As and other 2′-5′ oligonucleotides
Single-product OAS assay using dATP and A(pA)3 as substrates. Purified human recombinant His-OAS1 enzyme was incubated with dATP and A(pA)3 for 30 min at 37 °C, and the reactants and product resolved using a HiTrap Q column. In the figure, three separate chromatograms have been superimposed with the position of the respective molecules indicated. Blue curve: Profile from a reaction using His-OAS1 with dATP and A(pA)3 as substrates. Red and green curves: Control reactions using His-OAS1 together with dATP or A(pA)3 alone, respectively. Brown curve: Experimental salt gradient. Chromatograms were obtained with 254 nm as the absorbance wavelength. mAU, milli-absorbance unit and mS/cm, milliSiemens/centimeter