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Figure 5 | BMC Biochemistry

Figure 5

From: The androgen receptor plays a suppressive role in epithelial- mesenchymal transition of human prostate cancer stem progenitor cells

Figure 5

In vitro combinatory use of LY 294002 (inhibitor of AKT signaling molecules) with γ-TT and/or 5-AZA showed synergistic effect of killing PCa S/P cells. (A) Soft agar colony formation assay. A cell suspension (1 × 104 cells/well) in 2 ml of 0.5% low-melting agar with RPMI 1640 was overlaid into 6well plates containing 1% agar base. The 2 ml normal medium was added on the upper cells contained agar. The plates were incubated at 37°C in a humidified incubator for 21 days. The colonies were visualized by staining with 1% crystal violet. Colonies larger than 1 mm in diameter were counted. (B) MTT assay. Cells were incubated in 24 well dishes (1 × 104 cells/well). At 0, 2, 4, and 6 days, MTT assays were performed.

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