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Figure 3 | BMC Biochemistry

Figure 3

From: Nickel quercetinase, a “promiscuous” metalloenzyme: metal incorporation and metal ligand substitution studies

Figure 3

Far-UV CD spectra of QueD variants carrying amino acid replacements. (A), Ni-QueD proteins with amino acid substitutions in the 3His/1Glu motif proposed to ligate the metal ion, and (B) Co-QueD with a substitution of the glutamate ligand by histidine. Protein samples in 10 mM potassium phosphate buffer (pH 8.0) were adjusted to ~10 μM (based on measuring absorbance at 280 nm). Spectra were recorded at 25°C in a 1 mm path length cell; bandwidth: 0.1 nm.

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