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Table 4 Thermodynamics and kinetics of Orai-CMBD to CaM and chimeras

From: The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study

Protein

K a1 (M −1 )

ΔH 1 (kcal/ mol)

K a2 (M −1 )

ΔH 2 (kcal/ mol)

Binding model or N for one set of sites

k off (s −1 )

CaM

8.92 ± 1.03 × 105

- 5.02 ± 0.13

n.a.a

n.a.

N = 2.05 ± 0.30

1.41 ± 0.08

CaM(1TnC)

7.57 ± 1.10 × 105

- 4.99 ± 0.14

1.70 ± 0.30 × 104

- 3.33 ± 0.30

Sequential

2.44 ± 0.21

CaM(2TnC)

6.66 ± 0.20 × 105

- 5.21 ± 0.21

1.39 ± 0.35 × 104

- 3.79 ± 0.39

Sequential

2.64 ± 0.32

CaM(3TnC)

2.90 ± 0.12 × 105

- 6.58 ± 0.05

n.a.

n.a.

N = 1.93 ± 0.24

0.68 ± 0.25

CaM(4TnC)

5.77 ± 1.35 × 105

- 5.34 ± 0.40

n.a.

n.a.

N = 1.87 ± 0.15

0.72 ± 0.31

  1. aNot applicable.