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Table 2 ANS lifetime a for the uncomplexed and complexed CaM and chimeras

From: The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study

 

Without Orai -CMBD

With Orai- CMBD

 

Component 1

Component 2

Average lifetime (ns)

Component 1

Component 2

Average lifetime (ns)

 

Ï„ 1 (ns)

f 1

Ï„ 2 (ns)

f 2

<Ï„>

Ï„ 1 (ns)

f 1

Ï„ 2 (ns)

f 2

<Ï„>

Ca2+-CaM

6.4

0.44

13.1

0.53

9.0

6.8

0.20

16.9

0.66

13.1

Ca2+-CaM(1TnC)

6.2

0.21

17.8

0.70

14.4

6.3

0.31

17.3

0.57

12.7

Ca2+-CaM(2TnC)

5.9

0.20

17.7

0.68

13.7

5.8

0.18

17.3

0.71

12.9

Ca2+-CaM(3TnC)

4.4

0.29

12.1

0.51

7.4

4.3

0.30

16.2

0.57

11.7

Ca2+-CaM(4TnC)

5.1

0.19

12.9

0.70

8.6

6.0

0.16

17.1

0.72

13.5

  1. aData from the fitting are only shown as an average for clarity.
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BMC Biochemistry

ISSN: 1471-2091

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