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Figure 8 | BMC Biochemistry

Figure 8

From: The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca2+-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study

Figure 8

Stern -Volmer plot of Trp76 fluorescence of Orai- CMBD quenched by acrylamide. A representative figure of Stern-Volmer quenching by acrylamide is shown for Orai-CMBD and CaM/Orai-CMBD based on steady state fluorescence. To ensure no free Orai-CMBD peptide in the complex, CaM or the chimeras were added into the solution of 5 μM Orai-CMBD until no significant fluorescence change was observed. The values from the fitting are shown in Table 3. Insert: the quenching was monitored by two lifetime components of Trp76 of Orai-CMBD in the complex.

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BMC Biochemistry

ISSN: 1471-2091

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