Figure 3

Polyhedrin 58–110 and 1–110 produce electron dense nanoparticles. A, Representative transmission electron microscopy (TEM) of the segment of the cell nucleus from a Sf9 cell infected with a recombinant baculovirus expressing PH_(1–110)­EGFP. The bar scale indicates 1 micron and applies to all panels in the figure. B, representative scanning electron microscopy of purified nanoparticles produced by the expression of PH_(1–110)­EGFP. C, confocal microscopy of purified nanoparticles produced by the expression of PH_(1–110)­EGFP. D, identification of main nanoparticle sizes in solution using Nanoparticle Tracking Analysis (NTA). Notice that all nanoparticles identified are multiples of the smallest size identified with NTA of approximately 100 nm. Numbers next to each peak identify the mean peak nanoparticle size value in nanometers (nm). All nanoparticles were purified from Sf9 lysates and isolated by low speed centrifugation, as indicated in material and methods. Identical nanoparticles were observed when using PH_(58–110)­EGFP (data not shown).