Figure 2From: Human DNA-binding peptidyl-prolyl cis/trans isomerase Par14 is cell cycle dependently expressed and associates with chromatin in vivo Par14 binds to DNA in vivo and elutes with increasing salt concentrations. A) An asynchronous culture of HeLa cells was subjected to a biochemical fractionation of nuclear and nucleic acid binding proteins. B) Immunoblotting with the affinity-purified αPPIase, MEK2, and nuclear matrix protein p84 antisera to benchmark the different fractions at 1:1000 dilution.Back to article page