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Figure 1 | BMC Biochemistry

Figure 1

From: Human DNA-binding peptidyl-prolyl cis/trans isomerase Par14 is cell cycle dependently expressed and associates with chromatin in vivo

Figure 1

Par14 localizes more to chromatin than to nuclear matrix. A) An asynchronous culture of HeLa cells was subjected to biochemical fractionation. B) Immunoblotting with the affinity-purified αPPIase, αMEK2, αLamin B1 and αHistone H3 antisera to benchmark the different fractions at a 1:1000 dilution. C) Distribution of Par14 in the various fractions quantified by densitometric analysis. The area of the band signals from three independent experiments were measured, normalized and the background signals were subtracted. Multi Gauge densitometry software version 3.1 was used.

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BMC Biochemistry

ISSN: 1471-2091

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