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Figure 3 | BMC Biochemistry

Figure 3

From: Folding and self-association of atTic20 in lipid membranes: implications for understanding protein transport across the inner envelope membrane of chloroplasts

Figure 3

CD analysis of atTic20 and atTic20ΔN20 in detergents and liposomes. (A) Far-UV CD spectra of purified atTic20 and atTic20ΔN20 in 0.1% ZW 3-14 detergent. The protein concentration was ~10 μM, as determined by Bradford protein assay. (B) Far-UV CD of atTic20 in detergent and POPC lipid vesicles. Protein and lipid concentrations were ~ 500 nM and 0.5 mM, respectively. All samples were measured in buffer containing 20 mM Tris-HCl, 100 mM NaCl, 1% glycerol, and pH 8.0. Summary of deconvoluted data are shown in Table 1.

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