Table 2 Kinetic analysis of auto-ADP-ribosylation of various arginines on M8 derived mutants
From: ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates
|  | Km, biotin-NAD (μM) | Vmax (μM/min) |
|---|---|---|
M8(K506R) | 31.7 ± 28.2 | 21767 ± 700.8 |
M8(K519R) | 100 ± 121.8 | 1350 ± 997 |
M8(K525R) | 79.3 ± 110 | 1005 ± 779.6 |
M8(K540R) | *n.d. | *n.d. |
M8(K543R) | 15.1 ± 25 | 56.6 ± 27.9 |
M8(K566R) | n.d. | n.d. |