Figure 8
From: ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates
Poly-L-arginines enhanced biotin signals on the auto-ADP-ribosylated wild type CTc. (A) Various concentrations of free poly-L-arginines (Poly-L-Arg) or L-arginine (L-Arg) were added to the auto-ADP-ribosylation reaction of CTc and incubated at 37°C for 1 hr. (B) The biotinyl-auto-ADP-ribosylation signals were detected by Western blotting and presented as fold of change compared to the no amino acid added sample. The data shown is representative of three different experiments.