Figure 2

SP site availability semi-quantitatively controls neuronal differentiation activity of xNgn2 in vivo . (A) Embryos were injected into 1 cell of 2 cells with 20 pg of mRNA encoding GFP, xNgn2, or the indicated mutant version of xNgn2 from the cumulative mutant series (see Additional file 2: Figure S2), injected side to the left. Embryos were fixed at stage 15 and subject to in situ hybridization for neural ß-tubulin expression. (B) Scoring of ectopic neurogenesis on the injected side of embryos from (A) on a scale of −1 - +3 [38]. The experiment was performed in triplicate (n = 47-87).