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Figure 1 | BMC Biochemistry

Figure 1

From: Pro-protein convertases control the maturation and processing of the iron-regulatory protein, RGMc/hemojuvelin

Figure 1

A pro-protein convertase inhibitor prevents accumulation of soluble 40 kDa RGMc. A. Map of mouse RGMc with RGD motif, von Willebrandt factor (vWF) type D domain, GPI anchor, and intra-molecular cleavage site (arrow) indicated. The conserved PC site (underlined, arginines bolded) is aligned below the map for 7 mammalian species. B. Absence of putative PC sites in RGMa or b. Alignment of human RGMa, b, and c; vertical lines or asterisks denote identical residues between paralogous proteins. C. Immunoblot of affinity-purified RGMc from mouse serum and muscle cell conditioned medium, with 50 and 40 kDa isoforms indicated. D. Immunoblot of affinity-purified RGMc from blood from two humans (a, b) and two mice (c, d), with 50 and 40 kDa isoforms indicated. Soluble HA-RGMc is included as a reference. E. Immunoblot of RGMc from muscle cell conditioned medium (50 μl) incubated without or with the small molecule PC inhibitor, RVKR. F. Immunoblot of RGMc from conditioned medium (25 μl) of C2 myoblasts or Hep3B cells infected with Ad-HA-RGMc and incubated ± RVKR. For C – F, black arrows indicate 50 kDa RGMc and white arrows 40 kDa.

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