Figure 5
From: Prion protein insertional mutations increase aggregation propensity but not fiber stability
Sup35NM and SP5NM are efficiently cross-seeded by SP14NM seeds. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Reactions were seeded with 2.5% (w/w) of preformed sonicated fibers as indicated. Graphs are plotted as %RFU versus time and are representative of three experiments. (A) Fiber formation of Sup35NM. (B) Fiber formation of SP5NM.