Figure 4
From: Prion protein insertional mutations increase aggregation propensity but not fiber stability
The addition of preformed seeds causes a spike in SP14NM fiber formation. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Graphs are plotted as %RFU versus time and are representative of three experiments. (A) SP14NM unseeded (solid line) and SP14NM seeded (dotted line). (B) Another spike in Th-T fluorescence is observed after the addition of more monomer (2.5 μM) after the SP14NM fiber formation reached a plateau.