Figure 5From: Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteinsMutation of the Rad23 UBL domain impairs proteasomal targeting. Recombinant Sic1 was ubiquitinated in vitro, proteasomes lacking Rad23 were treated with epoxomycin, and the two were then incubated at 30°C in the absence or presence of purified Rad23 proteins for 45 minutes. Deubiquitination of Sic1 was then analyzed by Western blotting for Sic1.Back to article page