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Table 3 Kinetic characterization of MurF inhibition by MurFp1 with respect to D-Ala-D-Ala.

From: Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF

Kinetic parameters

[MurFp1] (μM)

Value

IC50 (μM)

 

250 ± 10

Vmax (nmol/min)

0

0.61 ± 0.01

 

75

0.49 ± 0.01

 

150

0.4 ± 0.01

 

300

0.36 ± 0.02

 

600

0.22 ± 0.01

Km (μM)

0

115 ± 10

 

75

100 ± 10

 

150

85 ± 5

 

300

140 ± 30

 

600

115 ± 25

Mixed α

 

0.85 ± 0.25

Mixed β

 

8.5 × 10-10 ± 0.6 × 10-10

Mixed Ki (μM)

 

420 ± 100

Non-competitive β

 

8 × 10-10 ± 0.7 × 10-10

Non-competitive Ki (μM)

 

370 ± 55

  1. Kinetic parameters were monitored with a pre-incubation of 30 min with MurF, MurFp1 and UDP-MurNac-Ala-Glu-meso-A2pm.