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Table 1 Phage titers obtained after each round of bio-panning using MurF and the 12-mer library

From: Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF

 

Phage input

Eluted phages

Elution (%)

Round 1

   

Gly-HCl

4 × 1010

8.2 × 106

2 × 10-2

Round 2

   

Gly-HCl

1.5 × 1011

4.1 × 107

2.7 × 10-2

Round 3

   

Gly-HCl

2 × 1013

1.1 × 107

5.5 × 10-5

ATP

 

1.3 × 107

6.5 × 10-5

D-Ala-D-Ala

 

5.6 × 106

2.8 × 10-5

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BMC Biochemistry

ISSN: 1471-2091

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