The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71

BMC Biochemistry

Table 1 Binding sites of FF domains

FF protein	FF domain	Target protein	Binding site	Ref.
Prp40	FF1	Clf1	GSTNIDILDLEELREYQRRKRTEYEGYLKRNRLD	[17]
Prp40	WW+FF1-2	RNAPII-CTD	YpSPTpSPS	[22]***
Prp40	FF1	Luc7	φ[FHL] × [KR] × [GHL] = FLGKIHLG	This study
Prp40	FF2-3	Snu71	NDVHY	This study
CA150	FF1,2,3**	Tat-SF1	(D/E)_2/5-F/W/Y-(D/E)_2/5 *	[23, 21]
CA150	FF5	RNAP-CTD	YpSPTpSPS	[20]
FBP11	FF1	RNAP-CTD	YpSPTpSPS	[16]
Rho-GAP	FF1-4	TFII-I	(N-terminal 90 amino acids)	[15]

* i.e., two of the five residues either preceding or following an aromatic residue are negatively charged glutamic acid or asparagine.
** each of the FF1, FF2, and FF3 bound with similar affinity.
*** this interaction required both WW and FF domains. Neither of them was able to bind to the CTD by itself (pS indicates phospho-Serine).

ISSN: 1471-2091