Figure 2
From: The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71
The FF1 domain of Prp40 binds to Luc7 in vitro. Various GST-Prp40FF domain fusion proteins (FF1, FF2; FF4, FF1-2, FF3-4) were tested for Luc7 binding in vitro. Top panel shows Coomassie blue-stained GST fusion proteins, while bottom row shows autoradiography of bound Luc7 protein. Luc7 bound to a construct containing FF1-2 domains as well as to the FF1 domain alone, but barely to FF2 alone. I, input; L, ladder (molecular mass marker).