Figure 3From: Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coliRecBD fusion enzymes unwind dsDNA and make a cut after Chi. DNA unwinding and Chi cutting activities of the fusion enzymes were measured using pBR322 χ + DNA substrate labeled with 5'-32P. DNA substrates (4.5 nM) were incubated with crude extracts at 3.5 mM magnesium, monitored on 1% agarose gels and analyzed by autoradiography.Back to article page