Figure 2

Alignment of BDH sequences. Alignment of BDH sequences from mammalian species (rat, mouse, human and jerboa) with Pseudomonas aeruginosa (P. a.) was realized using ClustalW (Thompson et al., 1994). Identical and similar residues were shown in black and yellow background respectively. The presumed amino acids sequences corresponding to oligonucleotides used for the PCR amplification of Jerboa BDH cDNA are underlined. According to the identity between Rat, Mouse and Human, they are considered as putative sequences in Jerboa. Amino acids of the catalytic tetrad Asn111, Ser139, Tyr152 and Lys156 (P. a. numbering) are marked by a star (*). These amino acids correspond to Asn114, Ser142, Tyr155 and Lys159 of the Pseudomonas fragi BDH (Ito et al., 2006). Amino acids participating to the NAD⁺ binding pocket Gly12, Leu61, Ala88, Ile90 and Ile108 (P. a. numbering) are marked by a hash sign (#). These amino acids correspond to Gly11, Leu64, Ala91, Ile93 and Leu113 of the Pseudomonas fragi BDH (Ito et al., 2006).