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Table 2 Effects of L-arginine on synthase and kinase activities of the arginine resistant and wild-type XcNAGS-K.

From: Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapods

  Synthase Activity (μmol min-1mg-1) Kinase Activity (μmol min-1mg-1)
XcNAGS-K   +1 mM Arginine   +1 mM Arginine
Wild-type 71.00 ± 1.07a ndb 61.93 ± 0.83 7.52 ± 1.26
F35C 9.66 ± 0.25 4.31 ± 0.01 65.14 ± 4.77 44.35 ± 2.70
E280A 26.93 ± 0.68 26.68 ± 0.50 82.13 ± 1.79 82.44 ± 0.16
G286P 3.51 ± 0.04 3.39 ± 0.08 76.57 ± 3.97 50.70 ± 7.43
G288S 46.04 ± 1.09 42.55 ± 1.02 55.62 ± 1.26 42.92 ± 5.42
  1. aActivities are averages of three measurements and their associated standard errors
  2. bNot detectable.