Figure 3From: Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapodsPurification of wild-type and arginine-insensitive NAGS. Wild-type and mutant mouse NAGS (A) and X. campestris NAGS-K (B) were overexpressed in E. coli and purified using nickel-affinity chromatography.Back to article page