Figure 5
From: YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules
YB-1 promotes microtubule assembly and co-sediments with microtubule pellets. A, Turbidimetry plot of 20 μM tubulin assembly in the absence (a) or presence of increasing concentrations of YB-1 [2.5 μM (b), 5 μM (c) and 10 μM (d)] in buffer M with 10% glycerol. B, 20 μM tubulin was polymerized in buffer M with 10% glycerol in the absence (ctrl) or presence of 5 μM or 10 μM YB-1. After polymerization until steady-state, the samples were centrifuged, then supernatants and pellets were analyzed by SDS-PAGE. (S, supernatant; P, pellet). (Additional file 4).