Figure 3From: YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubulesYB-1:tubulin cross-linking. YB-1 and tubulin were cross-linked with EDC and sulfo-NHS, resolved by 9% SDS-PAGE, blotted and probed with anti-alpha, anti-beta tubulin and anti-YB-1 antibodies as described under Materials and Methods. Gels were either stained with Coomassie blue (top) or processed for Western blotting with the indicated antibodies.Back to article page