Figure 10
From: YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules
Tubulin modifies the structure of YB-1-RNA complexes. 30 nM Luciferase RNA was incubated in buffer M with 5% glycerol alone (lanes 1 and 2) or in the presence of 2.1 μM YB-1 (lanes 3, 4 and 5) for 10 min at 25°C. After incubation, 2.1 μM tubulin (lane 4) or 4.2 μM tubulin (lanes 2 and 5) were added and mixtures were analyzed by native agarose gel electrophoresis followed by staining with ethidium bromide.