Table 3 Primers used for mutagenesis

Ser33→Ala

Forward 5'-CGATATGTACGGTGCG^aAATAACCTTCAATTTTC-3'

Reverse 5'-GAAAATTGAAGGTTATTCGC ACCGTACATATCG-3'

Ser33→Trp

Forward 5'-CGATATGTACGGTTGG AATAACCTG^bCAATTTTC-3'

Reverse 5'-GAAAATTGCAG GTTATTCCA ACCGTACATATCG-3'

Trp70→Ala

Forward 5'-GAAATTTAACCAGGCG AGTGGCACATCTG-3'

Reverse 5'-CAGATGTGCCACTCGC CTGGTTAAATTTC-3'

Trp231→Ala

Forward 5'-GTTATCCATGAT CCGGCG gcagcttatc-3'

Reverse 5'-GATAAGCTGCCGC CGGATC ATGGATAAC-3'

Trp231→Phe

Forward 5'-GGTTATCCATGACCCGTTT GCAGCTTATCAG-3'

Reverse 5'-CTGATAAGCTGCAAA CGGGTCATGGATAACC-3'

Tyr245→Trp

Forward 5'-CAGGTCATGAATGG AGCACGCCAATCAAG-3'

Reverse 5'-CTTGATTGGCGTGCTCCA TTCATGACCTG-3'