Protein | Specific hydrolyzing activity (U/μmol protein)a | ||
---|---|---|---|
Crystalline chitin | Colloidal chitin | p NP-[GlcNAc]2 | |
Wild-type | 0.59 ± 0.02(100)b | 12.9 ± 0.22 (100) | 50.5 ± 1.13 (100) |
S33A | n.d.c | 8.5 ± 0.50 (66) | 58.0 ± 1.08 (115) |
S33W | 1.00 ± 0.08 (166) | 15.3 ± 0.32 (119) | 54.0 ± 2.55 (107) |
W70A | n.d. | 4.3 ± 0.17 (33) | 52.8 ± 2.14 (105) |
W231A | n.d. | 6.6 ± 0.26 (51) | 45.2 ± 2.00 (90) |
W231F | n.d. | 9.2 ± 0.49 (71) | 54.3 ± 2.85 (108) |
Y245W | 1.49 ± 0.09 (250) | 19.6 ± 0.53 (152) | 53.6 ± 1.61 (106) |