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Figure 6 | BMC Biochemistry

Figure 6

From: Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog

Figure 6

Co-localization of 8-[ϕ-575]-cAMP and GFP-tagged R subunits. COS-7 cells were transiently transfected with GFP-hRIα (a-c) and GFP-hRIIα (d-f), incubated with 500 μM 8-[ϕ-575]-cAMP for 30 minutes. Cells were fixed and fluorescence was imaged using confocal microscopy: (a,c) green fluorescence of GFP (b,e), red fluorescence of 8-[ϕ-575]-cAMP, (c,f) merged images. The scale bar indicates 10 μm.

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