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Table 3 Dissociation equilibrium constant of wild-type RNase and K41R for PLP, PMP, PPL-L-alanine and PPL-D-alanine

From: Molecular evolution of B6 enzymes: Binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme

 

K'da (μM)

Ligand

Wild-type

K41R

PLP

120 ± 5

140 ± 10

PMP

106 ± 5

107 ± 3

PPL-L-alanine

23 ± 1

23 ± 1

PPL-D-alanine

45 ± 1

40 ± 3

  1. aThe constants were determined by the quenching of the intrinsic fluorescence of RNase at pH 7.5 upon addition of the ligand (see Methods) as shown in Fig. 5 for RNase(K41R) and PPL-L-alanine. Results are the mean ± SE of three independent measurements.
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BMC Biochemistry

ISSN: 1471-2091

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