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Figure 5 | BMC Biochemistry

Figure 5

From: Molecular evolution of B6 enzymes: Binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme

Figure 5

Fluorescence titration of RNase(K41R) with PPL-L-alanine. Fluorescence was measured with 5 μM RNase(K41R) in 50 mM 4-methylmorpholine pH 7.5 at 25°C. PPL-L-alanine concentrations were 10, 12, 15, 20, 25, 40 and 50 μM. A, Fluorescence spectra; B, nonlinear regression analysis gives a K'd value of 23 μM. Bovine RNase A contains six tyrosine residues and no tryptophan residue [45].

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BMC Biochemistry

ISSN: 1471-2091

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