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Figure 4 | BMC Biochemistry

Figure 4

From: Molecular evolution of B6 enzymes: Binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme

Figure 4

β-Decarboxylation and racemization of L-aspartate by RNase(K41R)-PLP. Recombinant RNase(K41R)-PLP (▲, —) and wild-type RNase-PLP (●, --) were incubated with 20 mM L-aspartate in 50 mM 4-methylmorpholine pH 7.5 at 25°C. The concentration of RNase-PLP adduct was 11 mM in the case of the wild-type enzyme and 7 mM in the case of K41R. Samples of 20 μl were withdrawn at the indicated times and analyzed by derivatization with Marfey reagent and subsequent HPLC as described under Materials and Methods. The mean values ± SE of 5 different experiments with standard deviations are shown.

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BMC Biochemistry

ISSN: 1471-2091

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