Figure 3From: Molecular evolution of B6 enzymes: Binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzymeRacemization of L-alanine by RNase-PLP adduct. RNase-PLP (11 mM) was incubated with 20 mM L-alanine in 50 mM 4-methylmorpholine pH 7.5 at 25°C. Samples (20 μl) were withdrawn at the indicated times and analyzed by derivatization with Marfey reagent and subsequent HPLC (for details, see Materials and Methods). The mean values ± SE of 5 different experiments are shown. Similar data were obtained with D-alanine as substrate.Back to article page