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Figure 7 | BMC Biochemistry

Figure 7

From: Modulation of the pharmacological effects of enzymatically-active PLA2 by BTL-2, an isolectin isolated from the Bryothamnion triquetrum red alga

Figure 7

Male Wistar rats (120 g–150 g) were anaesthetised with halothane (inhaled). The paw oedema was induced by a single subplantar injection of 100 μL of sample 100 μg/paw). Paw volume was measured immediately before the injection and the intervals thereafter (15, 30, 60 and 120 minutes) using a hydroplethysmometer (model 7150, Ugo Basile, Italy). 7a) BTL-2 induced similar oedema simihat of the saline control. PLA2 showed the highest oedema at 30 minutes before the injection. The presence of BTL-2 decreased the oedema induced by the enzyme. 7b) The compound 48/80 (C48/80) was used, incubated with BTL-2, to show that the lectin was not acting on the tissues to decrease the action of PLA2, but was probably binding to the enzyme. Thus, the lectin did not decrease the action of C48/80 act.

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